The Karachi Marathon route commences at Nishan-e-Pakistan and proceeds eastbound on Sea View Road towards Clock Tower. Continuing onto Abdul Sattar Edhi Avenue, runners will pass Emaar.

The route then merges onto Do Darya Road, maintaining a southeasterly direction. At Creek Marina Towers, participants will execute a sharp right turn.

A U-turn will be performed at the Khayaban-e-Shajar intersection, directly in front of DHA Golf Club. The return journey will follow the same route, culminating back at Nishan-e-Pakistan.